New insights into Staphylococcus aureus stress tolerance and virulence regulation from an analysis of the role of the ClpP protease in the strains Newman, COL, and SA564.
Identifieur interne : 001C93 ( Main/Exploration ); précédent : 001C92; suivant : 001C94New insights into Staphylococcus aureus stress tolerance and virulence regulation from an analysis of the role of the ClpP protease in the strains Newman, COL, and SA564.
Auteurs : Dorte Frees [Danemark] ; Julie Hove Andersen ; Lene Hemmingsen ; Kerttu Koskenniemi ; Kristoffer T. B K ; Musemma Kedir Muhammed ; Dereje Dadi Gudeta ; Tuula A. Nyman ; Antti Sukura ; Pekka Varmanen ; Kirsi SavijokiSource :
- Journal of proteome research [ 1535-3907 ] ; 2012.
Descripteurs français
- KwdFr :
- Endopeptidase Clp (génétique), Expression des gènes, Facteurs de virulence (génétique), Phénotype, Prophages, Protéines bactériennes (génétique), Protéines bactériennes (isolement et purification), Protéines bactériennes (métabolisme), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Protéines de transport (génétique), Protéines de transport (métabolisme), Protéome (génétique), Protéome (isolement et purification), Protéome (métabolisme), Régulation de l'expression des gènes bactériens, Staphylococcus aureus (enzymologie), Staphylococcus aureus (génétique), Staphylococcus aureus (métabolisme), Staphylococcus aureus (physiologie), Stress physiologique, Techniques de knock-out de gènes, Transactivateurs (génétique), Transactivateurs (métabolisme), Urease (métabolisme), Virulence, Électrophorèse bidimensionnelle différentielle sur gel.
- MESH :
- enzymologie : Staphylococcus aureus.
- génétique : Endopeptidase Clp, Facteurs de virulence, Protéines bactériennes, Protéines de liaison à l'ADN, Protéines de transport, Protéome, Staphylococcus aureus, Transactivateurs.
- isolement et purification : Protéines bactériennes, Protéome.
- métabolisme : Protéines bactériennes, Protéines de liaison à l'ADN, Protéines de transport, Protéome, Staphylococcus aureus, Transactivateurs, Urease.
- physiologie : Staphylococcus aureus.
- Expression des gènes, Phénotype, Prophages, Régulation de l'expression des gènes bactériens, Stress physiologique, Techniques de knock-out de gènes, Virulence, Électrophorèse bidimensionnelle différentielle sur gel.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (isolation & purification), Bacterial Proteins (metabolism), Carrier Proteins (genetics), Carrier Proteins (metabolism), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Endopeptidase Clp (genetics), Gene Expression, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Phenotype, Prophages, Proteome (genetics), Proteome (isolation & purification), Proteome (metabolism), Staphylococcus aureus (enzymology), Staphylococcus aureus (genetics), Staphylococcus aureus (metabolism), Staphylococcus aureus (physiology), Stress, Physiological, Trans-Activators (genetics), Trans-Activators (metabolism), Two-Dimensional Difference Gel Electrophoresis, Urease (metabolism), Virulence, Virulence Factors (genetics).
- MESH :
- chemical , genetics : Bacterial Proteins, Carrier Proteins, DNA-Binding Proteins, Endopeptidase Clp, Proteome, Trans-Activators, Virulence Factors.
- chemical , isolation & purification : Bacterial Proteins, Proteome.
- chemical , metabolism : Bacterial Proteins, Carrier Proteins, DNA-Binding Proteins, Proteome, Trans-Activators, Urease.
- enzymology : Staphylococcus aureus.
- genetics : Staphylococcus aureus.
- metabolism : Staphylococcus aureus.
- physiology : Staphylococcus aureus.
- Gene Expression, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Phenotype, Prophages, Stress, Physiological, Two-Dimensional Difference Gel Electrophoresis, Virulence.
Abstract
In Staphylococcus aureus, ClpP proteases were previously shown to be essential for virulence and stress tolerance in strains derived from NCTC8325. Because these strains exhibit a severely reduced activity of the alternative sigma factor, SigB, we here reassessed the role of ClpP in SigB-proficient clinical strains. To this end, clpP was deleted in strains COL, Newman, and SA564, and the strains were characterized phenotypically. The proteomic changes accomplished by the clpP deletion in the different strains were analyzed using the 2-D DIGE technique. The proteomic analyses revealed mostly conserved changes in the protein profiles of the ClpP-deficient strains. Among the strain-specific changes were the up-regulation of prophage proteins that coincided with an increased spontaneous release of prophages and the relatively poorer growth of the clpP mutants in some strain backgrounds. Interestingly, the effect of ClpP on the expression of selected virulence genes was strain-dependent despite the fact that the expression of the global virulence regulators RNAIII, mgrA, sarZ, sarR, and arlRS was similarly changed in all clpP mutants. ClpP affected the expression of sarS in a strain-dependent manner, and we propose that the differential expression of sarS is central to the strain-dependent effect of ClpP on the expression of virulence genes.
DOI: 10.1021/pr200956s
PubMed: 22112206
Affiliations:
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(genetics)</term><term>DNA-Binding Proteins (metabolism)</term><term>Endopeptidase Clp (genetics)</term><term>Gene Expression</term><term>Gene Expression Regulation, Bacterial</term><term>Gene Knockout Techniques</term><term>Phenotype</term><term>Prophages</term><term>Proteome (genetics)</term><term>Proteome (isolation & purification)</term><term>Proteome (metabolism)</term><term>Staphylococcus aureus (enzymology)</term><term>Staphylococcus aureus (genetics)</term><term>Staphylococcus aureus (metabolism)</term><term>Staphylococcus aureus (physiology)</term><term>Stress, Physiological</term><term>Trans-Activators (genetics)</term><term>Trans-Activators (metabolism)</term><term>Two-Dimensional Difference Gel Electrophoresis</term><term>Urease (metabolism)</term><term>Virulence</term><term>Virulence Factors (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Endopeptidase Clp (génétique)</term><term>Expression des gènes</term><term>Facteurs de virulence 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gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In Staphylococcus aureus, ClpP proteases were previously shown to be essential for virulence and stress tolerance in strains derived from NCTC8325. Because these strains exhibit a severely reduced activity of the alternative sigma factor, SigB, we here reassessed the role of ClpP in SigB-proficient clinical strains. To this end, clpP was deleted in strains COL, Newman, and SA564, and the strains were characterized phenotypically. The proteomic changes accomplished by the clpP deletion in the different strains were analyzed using the 2-D DIGE technique. The proteomic analyses revealed mostly conserved changes in the protein profiles of the ClpP-deficient strains. Among the strain-specific changes were the up-regulation of prophage proteins that coincided with an increased spontaneous release of prophages and the relatively poorer growth of the clpP mutants in some strain backgrounds. Interestingly, the effect of ClpP on the expression of selected virulence genes was strain-dependent despite the fact that the expression of the global virulence regulators RNAIII, mgrA, sarZ, sarR, and arlRS was similarly changed in all clpP mutants. ClpP affected the expression of sarS in a strain-dependent manner, and we propose that the differential expression of sarS is central to the strain-dependent effect of ClpP on the expression of virulence genes.</div></front></TEI><affiliations><list><country><li>Danemark</li></country></list><tree><noCountry><name sortKey="Andersen, Julie Hove" sort="Andersen, Julie Hove" uniqKey="Andersen J" first="Julie Hove" last="Andersen">Julie Hove Andersen</name><name sortKey="B K, Kristoffer T" sort="B K, Kristoffer T" uniqKey="B K K" first="Kristoffer T" last="B K">Kristoffer T. B K</name><name sortKey="Gudeta, Dereje Dadi" sort="Gudeta, Dereje Dadi" uniqKey="Gudeta D" first="Dereje Dadi" last="Gudeta">Dereje Dadi Gudeta</name><name sortKey="Hemmingsen, Lene" sort="Hemmingsen, Lene" uniqKey="Hemmingsen L" first="Lene" last="Hemmingsen">Lene Hemmingsen</name><name sortKey="Koskenniemi, Kerttu" sort="Koskenniemi, Kerttu" uniqKey="Koskenniemi K" first="Kerttu" last="Koskenniemi">Kerttu Koskenniemi</name><name sortKey="Muhammed, Musemma Kedir" sort="Muhammed, Musemma Kedir" uniqKey="Muhammed M" first="Musemma Kedir" last="Muhammed">Musemma Kedir Muhammed</name><name sortKey="Nyman, Tuula A" sort="Nyman, Tuula A" uniqKey="Nyman T" first="Tuula A" last="Nyman">Tuula A. Nyman</name><name sortKey="Savijoki, Kirsi" sort="Savijoki, Kirsi" uniqKey="Savijoki K" first="Kirsi" last="Savijoki">Kirsi Savijoki</name><name sortKey="Sukura, Antti" sort="Sukura, Antti" uniqKey="Sukura A" first="Antti" last="Sukura">Antti Sukura</name><name sortKey="Varmanen, Pekka" sort="Varmanen, Pekka" uniqKey="Varmanen P" first="Pekka" last="Varmanen">Pekka Varmanen</name></noCountry><country name="Danemark"><noRegion><name sortKey="Frees, Dorte" sort="Frees, Dorte" uniqKey="Frees D" first="Dorte" last="Frees">Dorte Frees</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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